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Figure 3 | Retrovirology

Figure 3

From: HTLV-1 and -2 envelope SU subdomains and critical determinants in receptor binding

Figure 3

Intracellular expression of HTLV-1 Env chimeras and soluble SU subdomains. Cell extracts (A, B) or culture supernatants (C) were prepared from 293T cells transfected with either full length Env (A) or soluble SU subdomains (B, C) expression vectors as depicted in figure 2. Membranes were probed with either (A) an anti-MLV SU antiserum to detect F-MLV and H1183FEnv uncleaved Env precursor proteins (F-MLV Prgp85 and H1183Fenv Pr, respectively) indicated by arrowheads, and cleaved SU (F-MLV SUgp70 and H1183FEnv SU, respectively) indicated by circles, or (B, C) an anti-rabbit IgG antiserum to detect carboxy terminal rFc-tagged soluble subdomains, including the Ampho-MLV SU subdomain (A397SU).

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