Figure 2
From: HTLV-1 and -2 envelope SU subdomains and critical determinants in receptor binding
Schematic representation of HTLV/MLV Env chimeras and HTLV SU amino terminal subdomains. Env landmark positions are indicated and SU landmark sequences and positions are indicated by arrowheads. Open arrowheads indicate the position of construct borders. (A) HTLV/MLV Env chimeras. The H1215FEnv and H1183FEnv HTLV/MLV Env chimeras were obtained by replacing the 329 and 269 amino terminal residues of the F-MLV Env (open boxes) with the amino terminal 215 and 183 amino acid residues of the HTLV-1 Env (solid boxes), respectively. The H1215FEnv chimera, previously described and formerly designated HHproFc [9], has been renamed here for sake of nomenclature homogeneity. (B) Soluble HTLV-1 (H1) and HTLV-2 (H2) SU amino terminal subdomains, H1215SU, H2211SU, H1179SU, and H2178SU were constructed as fusion proteins with a carboxy terminal hemagglutinin (HA) or rabbit immunoglobulin Fc (rFc) tag. All amino acid residue numbering starts from the first methionine of the HTLV-1 or -2 Env signal peptide, the amino terminal 20 and 21 aa residues, respectively.