p30II activates NFAT, AP-1 and NF-κB transcriptional activity in Jurkat T lymphocytes. Black bars indicate control and grey bars indicate p30II. Data points are mean of triplicate experiments. Fold increase in activity in the presence of p30II is indicated above each bar. p30II increased the NFAT-luc activity from 2.2 to 10.7 fold depending on co-stimulatory treatment e.g., PMA, ionomycin, CD3, CD28 etc. (A), p30II increased NF-κB-luc activity from 3.1 to 11.4 fold (B) and modestly increased the AP-1 driven luciferase reporter gene activity from 1 to 5 fold in the presence of co-stimulator treatments (C).