Table 1 PCR primers used to amplify Mandrillus SIV-pol
Primer | Sequence ('5→'3) | Description | Fragment size |
|---|---|---|---|
SIVmnd1A | AGATATAGGGGATGCCTATT | 5' first primer | A-B = 356 nt |
SIVmnd1B | TCTTCCACTTATCTGGGTGT | 3' first primer | Â |
SIVmnd1C | AGATTATAGACCCTATACTGC | 5'second primer | C-D* = 282 nt |
SIVmnd1D | CATCCAATGAAAGGGAGGTTC | 3' second primer | Â |
SIVmnd2A | GGACATAGGGGATGCCTATT | 5' first primer | A-B = 356 nt |
SIVmnd2B | CTGTCCATTTCTTTGGGTGC | 3' first primer | Â |
SIVmnd2C | GGACTTTAGAAAGTACACTGC | 5'second primer | C-D* = 282 nt |
SIVmnd2D | CATCCACTCAAAGGGAGGTTC | 3' second primer | Â |
SIVdrlA | GGATGTAGGTGATGCCTATT | 5' first primer | A-B = 356 nt |
SIVdrlB | CTGTCCACTTCTTTGGATGC | 3' first primer | Â |
SIVdrlC | = SIVmnd2C | 5'second primer | C-D* = 282 nt |
SIVdrlD | CATCCATTCATAAGGAGGATTG | 3' second primer | Â |