Figure 3
Rescue by GagPol of tRNALys3 annealing in mutant virions. COS7 cells were transfected with either BH10P-, Δ467.P-, or Δ486.P-, and were also cotransfected with one of these plasmids and a plasmid coding for full-length GagPol (hGagPolΔFSΔPR). A. Western blots of cell lysates, probed with anti-RT or anti-β-actin. B. Western blots of viral lysates, probed with anti-RT and anti-CA. C. Incorporation of tRNALys3 into wild-type and mutant virions. Dot blots of viral RNA were hybridized with probes specific for tRNALys3 or genomic RNA, and the tRNALys3:genomic RNA ratios were determined by phosphorimaging. The values are the means +/- standard deviations of experiments performed three or more times. D,E. tRNALys3 annealing in wild-type and mutant virions. tRNALys3 annealing was measured as described in the Figure 2 legend. The values shown in E are the means +/- standard deviations of experiments performed three or more times.