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Figure 2 | Retrovirology

Figure 2

From: The connection domain in reverse transcriptase facilitates the in vivo annealing of tRNALys3 to HIV-1 genomic RNA

Figure 2

tRNALys3 annealing to viral genomic RNA. A. Total viral RNA was used as the source of primer tRNALys3/viral RNA template in an in vitro reverse transcription reaction as described in Methods. Six base extended tRNALys3 was resolved by 1D PAGE and quantitated by phosphorimaging. Each reaction used an equal amount of viral genomic RNA, as determined by hybridization with a genomic RNA-specific probe. B. Graphic presentation of 6 base-extended tRNALys3:genomic RNA ratios, normalized to BH10P-. The values are the means +/- standard deviations of experiments performed three or more times.

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