Figure 1
The incorporation of GagPol and tRNALys3into wild-type and mutant HIV-1. A. Schematic showing the deletions made in the Pol region of GagPol. Δ# designates the number of amino acid residues deleted from the C terminus of GagPol, and solid black lines represent the sequences not deleted. The RT sequence is divided into its known structural domains. The mutation D25G inactivates the viral protease. B. Western blots of viral lysates, probed with both anti-CA and anti-RT as previously described [18]. C. Incorporation of tRNALys3 into wild-type and mutant virions. Dot blots of viral RNA were hybridized with probes specific for tRNALys3 or genomic RNA, and the tRNALys3:genomic RNA ratios, normalized to BH10.P- were determined by phosphorimaging. The values are the means +/- standard deviations of experiments performed three or more times.