Table 2 In vivo follow up of transduced cells in blood. Absolute number of transduced cells per 10⁶ PBMCs were evaluated by semiquantitative PCR amplification of IFN-β transgene in the two groups of animals. For in vivo f ollow up of transduced cells in blood from macaques, DNA samples of PBMCs were obtained at different dates following infusion of transduced PBL. This table indicates the minimum and maximum number of days following the first infusion of transduced cells in which the construct used was still detectable in PBMCs. Moreover, maximum transduction rate of PBMCs and detection treshold of the PCR method are indicated in the two groups of animals. IFN group is represented by the three macaques that received their own cells transduced by the biologically active construct of IFN-β gene whereas the control group is represented by the three macaques that received their own cells transduced by the control one. The relative intensity of the signals was compared to serial dilutions of lysate derived from plasmid-transfected cells that contained known numbers of IFN-β transgene copy per cell. a Day 0 is the first infusion day, other infusions occured at days 361 and 613. b Absolute number of transduced cells was below 10 per 10⁶ PBMCs.

From: Low autocrine interferon beta production as a gene therapy approach for AIDS: Infusion of interferon beta-engineered lymphocytes in macaques chronically infected with SIVmac251