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Figure 6 | Retrovirology

Figure 6

From: Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex

Figure 6

DNA-tethered Tax is specifically active on the HTLV-I minimal promoter. (A) CAT reporter plasmid. Each plasmid contains five tandem copies of Gal4-binding sites and one copy of minimal promoter (TATAA) from adenovirus E1b, HIV-1 and HTLV-I. The minimal promoter sequences are shown in blue. (B) A representative example of CAT assay. The cells were co-transfected with 2 μg of a Gal4DB plasmid (pM vector alone for lanes 1–3, pGal4-VP16 for lanes 4–6, and pGal4-Tax for lanes 7–9) and 5 μg of a CAT reporter construct (pG5-E1B-CAT for lanes 1, 4 and 7; pG5-HIV-CAT for lanes 2, 5 and 8; and pG5-HTLV-CAT for lanes 3, 6 and 9). (C, D) Basal and activated transcriptional activities. HeLa cells were co-transfected with 5 μg of the indicated CAT reporter plasmids (pG5-E1B-CAT, pG5-HIV-CAT and pG5-HTLV-CAT) plus 2 μg of pM empty vector (C), pGal4-VP16 (D, blue) or pGal4-Tax (D, yellow). Basal CAT activity from pG5-HIV-CAT-transfected cells was taken as 100% (C, column 2).

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Retrovirology

ISSN: 1742-4690

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