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Figure 5 | Retrovirology

Figure 5

From: Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex

Figure 5

Tax preferentially activates the HTLV-I minimal TATAA promoter. (A) CAT reporter plasmid. Each plasmid contains two 21-bp repeats and one copy of minimal promoter (TATAA) from HTLV-I, HIV-1 and SV40. The minimal promoter sequences are shown in blue. (B) A representative example of CAT assay. The cells received 0, 0.5 and 1 μg of Tax-expressing plasmid pIEX and 5 μg of the indicated CAT reporter constructs (p21-HTLV-CAT, p21-HIV-CAT and p21-SV40-CAT). (C, D) Basal and Tax-induced transcriptional activities. HeLa cells were co-transfected with 5 μg of the indicated CAT reporter plasmids (p21-HTLV-CAT, p21-HIV-CAT and p21-SV40-CAT) plus 0.5 μg of pCMV empty vector (w/o Tax) or pIEX (w/ Tax). Basal CAT activity from p21-SV40-CAT-transfected cells was taken as 100% (C, column 3).

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