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Figure 6 | Retrovirology

Figure 6

From: The sequence of the CA-SP1 junction accounts for the differential sensitivity of HIV-1 and SIV to the small molecule maturation inhibitor 3-O-{3',3'-dimethylsuccinyl}-betulinic acid

Figure 6

Pulse-chase analysis of Gag processing in wild type and mutant HIV-1 and SIV particles. Virions were harvested at the indicated times from provirus-transfected 293T cells that were pulse-labeled with 35S-labeled amino acids and cultured in the presence and absence of DSB (2.5 μg/ml). Particles were lysed and the Gag proteins immunoprecipitated using CA-specific monoclonal antibodies, and radioactive proteins in the immunoprecipitates analyzed by SDS-PAGE and autoradiography. Panel A: Analysis of HIV-1 and HIVm2; Panel B: Analysis of SIV and SIVm3. Similar results were observed in two independent experiments.

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Retrovirology

ISSN: 1742-4690

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