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Figure 4 | Retrovirology

Figure 4

From: The sequence of the CA-SP1 junction accounts for the differential sensitivity of HIV-1 and SIV to the small molecule maturation inhibitor 3-O-{3',3'-dimethylsuccinyl}-betulinic acid

Figure 4

Mutations at the CA-SP1 junction confer DSB sensitivity to SIV, as revealed by single-cycle infection assays. Wild type and mutant SIV viruses were harvested from transfected 293T cells cultured in the presence of the indicated concentrations of DSB. Panel A: immunoblot analysis of viral lysates using a monoclonal antibody specific for SIV CA. Panel B: effects of DSB on SIV infectivity. Viral supernatants were assayed for infection of P4-CCR5 indicator cells. Infectivity was calculated after normalizing for the RT content of the SIV inocula. Values shown are normalized against the infectivity of the vehicle-treated virus stock. The absolute infectivity values of the control viruses are shown in Fig. 2D. The results are representative of two independent experiments.

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Retrovirology

ISSN: 1742-4690

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