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Figure 3 | Retrovirology

Figure 3

From: The sequence of the CA-SP1 junction accounts for the differential sensitivity of HIV-1 and SIV to the small molecule maturation inhibitor 3-O-{3',3'-dimethylsuccinyl}-betulinic acid

Figure 3

Mutations at the CA-SP1 junction render HIV-1 resistant to DSB, as revealed by single-cycle infection assays. Viruses were harvested from transfected 293T cells cultured in the presence of the indicated concentrations of DSB. Panel A: immunoblot analysis of viral lysates. Viral supernatants (1 ml) were pelleted, lysed, and subjected to SDS-PAGE and immunoblotting using polyclonal antisera to HIV-1 CA. Panel B: effects of DSB on HIV-1 infectivity. Viral supernatants were assayed for infection of P4-CCR5 indicator cells. Infectivity was calculated after normalizing for the p24 content of the inocula. Values shown are normalized against the infectivity of the respective vehicle-treated virus. The absolute infectivity values of the control viruses are shown in Fig. 2B. Results are representative of two independent experiments.

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Retrovirology

ISSN: 1742-4690

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