Table 1 Description of stable cell populations.a
From: Trans-dominant cellular inhibition of DC-SIGN-mediated HIV-1 transmission
Cell lines | Expression vectorsb | Antibiotic selection (concentration, μg/ml) |
|---|---|---|
Raji | NAc | NA |
Raji/DC-SIGN | pcDNA3-DC-SIGN | Neomycin (500) |
Raji/DN-CIITA | pDN-CIITA (300–1130) | Neomycin (500) |
Raji/DC-SIGN/DN-CIITA | pMX-DC-SIGN, pDN-CIITA (300–1130) | Neomycin (500) |
K562 | NA | NA |
K562/LFA-1 | pCDB1, pCDL1, pCMV-hph | Hygromycin (200) |
K562/DC-SIGN | pcDNA3-DC-SIGN | Neomycin (500) |
K562/DC-SIGN/LFA-1 | pcDNA3-DC-SIGN, pCDB1, pCDL1, pCMV-hph | Neomycin (500) Hygromycin (200) |
K562/WT-CIITA | pWT-CIITA | Neomycin (500) |
K562/WT-CIITA/LFA-1 | pWT-CIITA pCDB1, pCDL1, pCMV-hph | Neomycin (500) Hygromycin (200) |
K562/DC-SIGN/WT-CIITA | pBABE-DC-SIGN pWT-CIITA | Puromycin (1) Neomycin (500) |
K562/DC-SIGN/WT-CIITA /LFA-1 | pMX-DC-SIGN, pWT-CIITA, pCDB1, pCDL1, pCMV-hph | Neomycin (500) Hygromycin (200) |