Figure 4

Analysis of viral gene expression. (A) HHV-6B gene expression. Only the HHV-6 open reading frames are illustrated and indicated at the bottom of the panel. RNA extracted from HHV-6B-infected cells (Z29) and uninfected cells (SupT1) was used in hybridization assays. (B) HIV-1 gene expression and inhibition by CYC202. Only the HIV-1 open reading frames are illustrated and indicated at the bottom of the panel. The Tat and Rev coding sequences overlap in the HIV proviral genome. Primers were designed to amplify the first exon of Tat and the second exon of Rev (see Additional file 1). Both amplicons contain some overlap, with 84% of the Rev amplicon specific to Rev and 65% of the Tat amplicon specific to Tat. Tat and Rev indicated in the graph correspond to the amplicons that contain the majority of that particular sequence. Values were calculated and expressed as Log2 ratios. The red bars indicate hybridization in latent, uninduced cells; the green bars indicate genes that were expressed following TNF induction (20 ng/ml for 2 hrs). Both are compared to expression from uninfected cells. The blue bars indicate genes that were expressed following TNF induction compared to no induction; yellow bars indicate genes whose expression was effected by TNF and CYC202 (5 μM), as compared to untreated cells. (C) KSHV gene expression and inhibition by CYC202. Only the KSHV open reading frames are illustrated and indicated at the bottom of the panel. Values were calculated and expressed as mean Log2 ratios from four experiments. The yellow bars indicate genes that are expressed following TPA induction (20 ng/ml) while the red bars indicate genes whose expression was effected by TPA and CYC202 (5 μM).