Fig. 3
From: No evidence of bovine leukemia virus proviral DNA and antibodies in human specimens from Japan
Western blot analysis to test the anti-BLV reactivity of human serum. Human serum samples were used as primary antibody. M indicates protein molecular weight marker; + indicates FLK-BLV cell lysate applied on the SDS-PAGE; − indicates BLV-free FLK cell lysate applied on 12% SDS-PAGE; EBL cattle indicate serum obtained from BLV-infected with EBL; BLV (−) cattle indicates serum from cattle without BLV infection; No 1st Ab control indicates that membrane was incubated with 5% skim milk in PBS without any primary antibody