Fig. 5

Nuclear entry and integration of LV is reduced in iPSC. iPSC were transduced at an MOI of 10 (with independently produced viral supernatants as indicated) in the presence of 10 µM CSA or an equal volume of DMSO as solvent control. Cells were washed 6 h after vector application and harvested at indicated time points. Data are shown relative to the 24 h time point of LV transduced cells treated with CSA. Samples were analyzed for early RT (n = 3) (a), late RT products (n = 3) (b), 2-LTR circles (n = 6; * p = 0.030; ** p = 0.005) (c) and proviral vector copies (n = 3; ** p = 0.004; *** p ≤ 0.001) (d). RT products and 2-LTR circles were evaluated with TaqMan-based quantitative real-time PCR with the \(2^{{ - \Delta \Delta Ct}}\) method, normalized to endogenous PTBP2 copies. Proviral vector copies were determined by B1-LTR PCR and obtained values were corrected for plasmid contamination. Repeated measures one-way ANOVA with Tukey-Kramer post hoc test was used for statistical analyses. NTD non-transduced control; plasmid ctrl plasmid contamination control