Fig. 3

LDV co-infection is critical for the APOBEC3/Rfv3-dependent NAb response. B6 WT and mA3 KO mice were infected with 10⁴ SFFU of a FV/LDV or b LDV-free FV. Plasma samples at 28 dpi were heat-inactivated and the reciprocal plasma dilution that conferred 50% neutralization was computed. Log₄-transformed data are shown and used for statistical analyses. Each dot corresponds to a mouse and lines correspond to mean values. The total number of mice analyzed was combined from 2 independent experiments. Fold-change values in statistically-significant comparisons were based on median non-log-transformed values. Data were analyzed using 2-tailed unpaired Student’s t test, with p values indicated; ns, not significant (p > 0.05)