Fig. 3

Monocyte–macrophage frequencies during HIV-1 and ART treatments. Humanized NSG mice were uninfected, infected, or infected and treated with 2ART or 4ART regimens. Cells from blood, spleen and BM were identified as CD3−/CD20−/CD8−/HLA-DR+/CD14+CD16+ by flow cytometry, as described in methods. Data were analyzed with FlowJo software. Mean ± SEM for 4–9 mice per treatment group were compared by one-way ANOVA, and pairwise comparisons were determined by Fisher’s LSD post hoc test. P ≤ 0.05 compared with ^auninfected, ^binfected, or ^cinfected and treated with 2ART