Fig. 2
From: A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression
Modification of the C element abolishes gag and pol expression but C element is not essential for RNA export. a Subcellular distribution of foamy viral transcripts was analysed by northern blotting using a tas-specific probe. bet is expressed from a spliced transcript encompassing parts of the tas and bel2 gene [15, 20]. b Influences of the pol elements located in the SpeI- and SexAI-fragments on Gag expression were analysed by Western blotting with monoclonal anti-Gag antibodies. The position of the C element is indicated (blue box). Detection of GAPDH served as loading control. Samples were analysed in independent duplicates