Fig. 3
From: Evidence against a role for jaagsiekte sheep retrovirus in human lung cancer
PCR detection of JSRV sequences in the genomic DNA of cells exposed to live JSRV virus (LV), heat-inactivated virus (HI) or culture medium alone (−). PCR amplification procedures are described in “Methods”. M indicates a 1 kb Plus DNA Ladder (Thermo Fisher) with select band sizes indicated. The expected JSRV amplification product (arrows) has a size of 502 bp, which is clearly visible in the live virus (LV) lanes of all cell types except for the NIH 3T3 cells, which lack a functional cell-surface receptor for JSRV. This band is absent in all of the heat inactivated (HI) virus and negative control (−) lanes. The top two panels show an analysis of cellular DNA harvested 3 days after virus exposure, and this experiment was repeated once with identical results. The bottom two panels show an analysis of cellular DNA harvested 8 days after virus exposure, and this experiment was performed once