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Table 2 Binding parameters determined from FA salt-titration assays

From: Mechanistic differences between HIV-1 and SIV nucleocapsid proteins and cross-species HIV-1 genomic RNA recognition

RNA

HIV-1 NC

SIV NC

HIV-1 Gag∆p6

K d(1M) (M)a

Z beff

K d(1M) (M)a

Z beff

K d(1M) (M)a

Z beff

HIV-1 TARpolyA

(1.2 ± 1.2) × 10−4

2.4 ± 0.4

(8.2 ± 2.3) × 10−5

2.1 ± 0.1

(1.3 ± 0.1) × 10−1

8.7 ± 0.5

HIV-1 Psi

(4.0 ± 2.6) × 10−6

1.2 ± 0.1

(3.9 ± 1.5) × 10−6

1.3 ± 0.1

(1.8 ± 2.0) × 10−4

5.0 ± 2.0

SIV TAR

(8.1 ± 6.5) × 10−5

2.6 ± 0.4

(6.2 ± 4.3) × 10−5

2.4 ± 0.3

(1.2 ± 1.9) × 10−1

8.0 ± 1.4

SIV Psi

(1.0 ± 0.7) × 10−4

3.6 ± 0.9

(7.2 ± 3.1) × 10−5

3.0 ± 0.3

(4.7 ± 4.0) × 10−2

8.8 ± 1.0

  1. All values represent the average of at least three trials with the associated standard deviation
  2. aKd(1M) is the affinity at 1 M NaCl, which represents the specific, non-electrostatic component of binding
  3. bZeff is the number of Na+ ions released upon protein binding, and reflects the number of electrostatic contacts between protein and RNA