Fig. 2

Confirmation of the computer prediction model. a Growth kinetics of the mutants. At least three independent experiments were performed and similar results were obtained. One of the representative data for each mutant was shown. The molecular infectious clone, NL43 (the wild-type: WT) and its env mutant, NLNh, was put as positive and negative controls in each experiment respectively. The X-axis represented the days post infection (dpi), and the Y-axis represented the RT activity (PSL) in cell supernatant measured by Imaging Plate (Fujifilm, Japan). b Packaging efficiency of each mutant. The Y-axis represented the magnitude of efficiency of each mutant compared to that of the control vector, NLNh-CAmut (see “Methods” section). c Dimerization ability of each mutant. The Y-axis represented the magnitude of efficiency of each mutant compared to that of the wild-type (WT). The value of the WT was set as one. At least three independent experiments were performed. The data shown were the average of the value and error bars represented SEM. *Statistical significance compared to the wild-type (WT) at p < 0.05, and **statistical significance at p < 0.01