Fig. 7
From: HIV-1 capsid uncoating initiates after the first strand transfer of reverse transcription
Robustness of parameter fitting and testing the mathematical model of uncoating. a–d Estimated marginal distributions were computed for each model parameter from the calculated posterior distribution. Best fit parameters were defined using median values of marginal distribution and presented with 95 % credible intervals in (e). f The time of uncoating, tuncoating was estimated using the best fit parameters. g Testing the model predictions of infection kinetics in the CsA washout assay. OMK cells were infected with GFP-HIV VLP in the presence of CsA and 10 μM NVP for 2 h starting at 0, 1 or 2 h.p.i. CsA was removed from each sample at the indicated time. After 72 h, the percentage of GFP positive cells was measured for each sample and plotted relative to the percentage of GFP positive cells following infection in the absence of NVP when CsA was removed 7 h.p.i. Points represent experimental data from two independent experiments and solid lines indicate model predictions