Figure 1

Sequence alignment of A3H HapII (residues 1 to 183) and four A3 deaminase-active proteins whose structures have been solved: A3A (1 to 199) [ 41 ], A3C (1 to 190) [ 49 ], A3F-CTD (185–373) [ 54 , 55 ], and A3G-CTD (191–384) [ 53 ] (also see refs. [ 43 , 50 - 52 ]). The A3H residues mutated in this study are highlighted with green asterisks and the active site residues are highlighted in blue. The region inside the square brackets represents the Z domain sequences for all of the proteins. The amino acids that comprise A3H loop 7 are also shown. The sequence alignment was generated using Lasergene software (DNASTAR, Inc., Madison, WI, USA).