Figure 7
From: Interplay between the HTLV-2 Tax and APH-2 proteins in the regulation of the AP-1 pathway
APH-2 and Tax2B modulate the activity of the collagenase promoter. 293T cells were transfected with the reporter plasmid pCollagenase-Luc and the indicated expression vectors. Luciferase and Renilla activities were measured 2 days after transfection. The average of at least three different experiments is shown. The expression levels of Tax2B-His, c-Jun-Myc, FLAG-JunB and FLAG-APH-2 were visualized by Western blot using the indicated antibodies. (A) APH-2 suppresses Tax2B-induced collagenase transcription (B and C) APH-2 activates c-Jun and JunB-mediated transactivation of the collagenase promoter, respectively.