Figure 2
From: HRP-2 determines HIV-1 integration site selection in LEDGF/p75 depleted cells
HRP-2 overexpression rescues HIV-1 replication. WT and stable LEDGF/p75 KD cell lines (LEDGF/p75 KD) were complemented with LEDGF/p75 (LEDGF/p75 BC) or HRP-2 (LEDGF/p75 KD + HRP-2). (A) HRP-2 mRNA expression levels shown as fold overexpression compared to WT. (B) Relative luciferase activity (RLU/μg protein) following HIV-fLuc transduction. Data were compiled from at least six independent experiments and expressed as percentages relative to LEDGF/p75 BC (mean ± SD). (C) Multiple round HIV-1 replication after challenging the indicated cell lines with the laboratory strain HIV-1NL4.3. Replication was monitored by measuring the p24 content in the supernatant until day 10 at which point cells were confluent or showed massive cell death due to CPE. P24 levels decreased after day 8 in the WT condition due to CPE. Experiments were performed at least three times, a representative experiment is shown. (D) At day 10 of the experiment shown in (C), cells were split and maintained under antiretroviral therapy (AZT, 50xIC50) for 10 days to dilute all non-integrated viral DNA forms, before determining the number of integrated copies using qPCR.