Figure 4

Lentiviral vector sequences in human DC transduced with IDLV-M1, IDLV-M1/Vpx and LV-M1. At day 7 from transduction DNA was extracted and used in semi-quantitative Alu-PCR for evaluating integrated vector sequences (left panels, a representative experiment out of three performed is shown). The 293 cell line stably transduced with the TY2-GFP-IRES-Neo vector (293/LV-GFP-Neo) was used as standard for evaluating integrated vector copies. PCR samples were run on a Ethidium bromide stained 2% agarose gel. Real-time DNA PCR was used for evaluating 2-LTR and late reverse transcription products copy number (right panel, average values of three independent experiments are shown), as described in Materials and Methods.