Figure 3From: Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virusThe DD Env supports more efficient infection of HEK293T cells. (A) MLV(FeLV) lacZ pseudotypes were prepared from HEK293T cells. Samples of each pseudotype preparation were pelleted, separated by SDS-PAGE, immunoblotted and probed with either anti-FeLV gp70 or anti-SFFV Gag. (B) Matched inputs (RT activity) of MLV(FeLV) lacZ pseudotypes bearing the Glasgow-1 (A), DD, ND or NN Envs, were plated onto HEK293T cells. 72 hours post-infection, the cells were stained for expression of lacZ and counted manually. Values are presented as infectious units per μl (I.U./ μl ), mean +/− SE of three independent experiments. The increase in titre noted for the DD mutant and the decrease in titre of the ND mutant (asterisks) are statistically significant in comparison with A (Glasgow-1) (unpaired T test, p < 0.05).Back to article page