Nef functions in BLT mice to enhance HIV-1 replication and deplete CD4+CD8+ thymocytes
© Zou et al.; licensee BioMed Central Ltd. 2012
Received: 18 March 2012
Accepted: 28 May 2012
Published: 28 May 2012
The outcome of untreated HIV-1 infection is progression to AIDS and death in nearly all cases. Some important exceptions are the small number of patients infected with HIV-1 deleted for the accessory gene, nef. With these infections, disease progression is entirely suppressed or greatly delayed. Whether Nef is critical for high levels of replication or is directly cytotoxic remains controversial. The major problem in determining the role of Nef in HIV/AIDS has been the lack of tractable in vivo models where Nef’s complex pathogenic phenotype can be recapitulated.
Intravenous inoculation (3000 to 600,000 TCIU) of BLT humanized mice with HIV-1LAI reproducibly establishes a systemic infection. HIV-1LAI (LAI) replicates to high levels (peak viral load in blood 8,200,000 ± 1,800,000 copies of viral RNA/ml, range 3,600,000 to 20,400,000; n = 9) and exhaustively depletes CD4+ T cells in blood and tissues. CD4+CD8+ thymocytes were also efficiently depleted but CD4+CD8- thymocytes were partially resistant to cell killing by LAI. Infection with a nef-deleted LAI (LAINefdd) gave lower peak viral loads (1,220,000 ± 330,000, range 27,000 to 4,240,000; n = 17). For fourteen of seventeen LAINefdd-infected mice, there was little to no loss of either CD4+ T cells or thymocytes. Both LAI- and LAINefdd-infected mice had about 8% of total peripheral blood CD8+ T cells that were CD38+HLA-DR+ compared <1% for uninfected mice. Three exceptional LAINefdd-infected mice that lost CD4+ T cells received 600,000 TCIU. All three exhibited peak viral loads over 3,000,000 copies of LAINefdd RNA/ml. Over an extended time course, substantial systemic CD4+ T cell loss was observed for the three mice, but there was no loss of CD4+CD8+ or CD4+CD8- thymocytes.
We conclude Nef is necessary for elevated viral replication and as a result indirectly contributes to CD4+ T cell killing. Further, Nef was not necessary for the activation of peripheral blood CD8+ T cells following infection. However, CD4+CD8+ thymocyte killing was dependent on Nef even in cases of elevated LAINefdd replication and T cell loss. This depletion of thymic T cell precursors may be a significant factor in the elevated pathogenicity of CXCR4 trophic HIV-1.
KeywordsHIV-1 Nef Humanized mouse Replication Pathogenicity
HIV-1 infection leads to the near total loss of CD4+ T cells and results in immune incompetence [1, 2]. Nef is considered to be a critical inducer of pathogenicity for HIV-1, because there are several reported cases of human infection by HIV-1 lacking a functional nef that failed to develop AIDS for twelve years or more [3–9]. Also, support for an important role for simian immunodeficiency virus Nef in pathogenesis and disease progression comes from elegant experiments performed in non-human primates where the absence of Nef resulted in delayed disease progression [10, 11].
In vivo and ex vivo models of HIV-1 infection have been utilized to assess the role of Nef in viral replication and pathogenesis. Transgenic mouse models have demonstrated that Nef is the only HIV-1 protein that has direct pathogenic effects in mice [12–14]. Results from an HIV-1 infection model employing ex vivo cultures of human tonsil suggested a role for Nef as a replication factor [15–18]. Ex vivo experiments with human fetal thymus organ culture (HF-TOC) found that nef functioned as a pathogenic factor that does not enhance replication . The findings with HF-TOC were confirmed with the SCID-hu thy/liv implant model in which infection can be extended beyond the maximum two-week duration for most ex vivo models . Other groups found a dual role for Nef in HIV-1 infection of SCID-hu thy/liv implants as a replication and a pathogenesis factor [20, 21]. Aldrovandi et al. found the impact of nef-deleted HIV-1 on viral replication to be dependent on the initial dose [22, 23].
To address Nef’s role in HIV-1 pathogenesis during prolonged systemic infection, we have investigated the impact of inactivating nef on HIV-1 infection in vivo using BLT humanized mice [24–27]. This advanced model for human immune system reconstitution combines human stem cell engraftment in bone marrow with a human fetal liver/thymus implant producing a full range of systemically disseminated human immune cells including B cells, monocytic cells, dendritic cells and T cells. Human thymocyte education occurs within the implanted thymus which is a fully human cellular compartment [24, 26, 28]. BLT humanized mice have both human T cells and human thymocytes that can be infected simultaneously. This distinction establishes the BLT mouse model as a novel system for determining in vivo pathogenesis attributable to HIV-1 accessory genes. We inoculated mice with the strictly CXCR4-tropic virus, HIV-1LAI (LAI), to maximize the pathogenic impact of the infection [29, 30]. At three different inoculums in BLT mice, LAI rapidly depletes human CD4+ T cells in the peripheral blood and in tissues and eliminates CD4+CD8+ thymocytes from the implanted human thymic tissue .
At a low intravenous inoculum, we found nef-defective LAI to be greatly delayed for replication with lower peak viral loads relative to wild-type LAI. There was minimal CD4+ T cell and CD4+CD8+ thymocyte killing. At a high dose, the delay in nef-defective LAI replication was largely lost and in a few exceptional cases there was gradual loss of CD4+ T cells in blood and tissues. However, in no case did nef-defective LAI infection result in loss of thymocytes.
With these findings we have established the humanized BLT mouse model as highly appropriate for directly studying the complex role of Nef in processes that are critical for disease progression during HIV-1 infection. Our results suggest an indirect role for Nef in T cell killing and a direct role for Nef in loss of thymic function in HIV-1 infection [19, 31, 32].
Defining the course of infection in the BLT humanized mouse with the highly pathogenic HIV-1LAI
After establishing the effect of LAI infection on peripheral blood CD4+ T cell levels, we evaluated its effect on CD4+ cell depletion in lymph nodes, spleen and in the human thymic organoid (HTO). LAI depleted CD4+ T cells from lymph node and spleen at all doses (Figure 1C). The CD4+CD8+ double positive thymocytes were also efficiently depleted (Figure 1C). Interestingly, we noted that CD4+CD8- single positive cells in the thymus were relatively resistant to depletion with only the highest inoculum significantly decreasing the level of these cells (Figure 1C). After evaluating the dose–response relationship of LAI and establishing its pathogenic potential in BLT mice, we next investigated the role of Nef.
Nef-defective LAI, LAINefdd
Infection of BLT mice with a low dose of LAINefdd
To further explore the impact of nef-defective HIV infection, CD4+ T cells in lymphocyte containing tissues were analyzed. As seen in Figure 3C, LAINefdd infection did not result in depletion of CD4+ T cells in lymph node (LN), spleen, bone marrow (BM), lung and liver reflecting what was observed in peripheral blood (Figure 3B and C). Consistent with the fact that at this low dose wild-type LAI did not induce a severe depletion of CD4+CD8- thymocytes, infection with the LAINefdd also did not result in CD4+CD8- thymocyte depletion (Compare Figure 1C and 3C). However, a dramatic difference between LAI and LAINefdd infections was noted in the levels of CD4+CD8+ thymocytes present in the implanted human thymus. Specifically, infection with wild-type virus resulted in a severe depletion of double positive thymocytes, but infection with the nef-deleted virus did not deplete these cells (Figure 3C). These data indicate that 3000 TCIU of LAINefdd is sufficient to establish an infection in BLT humanized mice. However, consistent with Nef’s role as an important replication and pathogenic factor LAINefdd was greatly reduced in its ability to initiate replication and to induce systemic CD4+ T cell and double-positive thymocyte depletion.
Infection of BLT mice with an intermediate dose of LAINefdd
We then examined the levels of systemic CD4+ T cell depletion in the mice receiving this higher dose of virus. In the mice injected with LAI there was a dramatic depletion of CD4+ T cells in the lymph node, spleen, bone marrow, lung, liver and double positive thymocytes (Figure 4C). Importantly, CD4+ T cells were preserved in all the tissues analyzed from the mice infected with the nef-defective virus including the human thymic organoid (Figure 4C). These results demonstrate that, even with 10-fold higher inoculums, nef-deleted LAI is still highly attenuated in its ability to deplete T cells and thymocytes. However, the delay in viral replication observed in Figure 4A appeared to be less dramatic at this higher dose for LAINefdd-5 and LAINefdd-7 than for LAINefdd infection at 3000 TCIU (Figure 3A). The extreme delay in LAINefdd-6 will be considered in the Discussion.
Infection of BLT mice with a high dose of LAINefdd
At eight weeks post infection, we sacrificed the four LAINefdd- and three LAI-infected mice to determine the levels of CD4+ T cells in tissues (Figure 5C). Systemically, infection with wild type LAI at this high dose resulted in near total depletion of CD4+ T cells in all tissues analyzed and double-positive thymocytes as well. Interestingly, single positive CD4+ thymocytes did not appear to be fully depleted by LAI (Figure 5C). Mice infected with LAINefdd at this higher dose, maintained normal levels of CD4+ T cells and double-positive thymocytes. These results demonstrate that over a 200-fold range in the dose of virus, Nef is necessary for rapid loss of CD4+ T cells and CD4+CD8+ thymocytes. A role for Vpr, Env or possibly other HIV-1 proteins is not excluded, but all of these genes are functional in LAI and LAINefdd[42–44]. A recent report found no impact of deleting vpu on CD4+ T cell depletion by HIV-1AD8+ in humanized mice .
Infection of BLT mice with a high dose of LAINefdd for an extended time course
We considered the possibility that LAINefdd-12, LAINefdd-13 and LAINefdd-14 exhibited T cell depletion because rearrangements of the remnants of nef coding sequence may generate a severely truncated nef open reading frame with some restoration of function. In Additional file 3: Figure S3, we have aligned the sequences for the nef regions obtained by RT-PCR from the terminal bleed of these mice. All three of the sequences are identical to the sequence of the input virus. Therefore, nef from these three mice remained non-functional.
In Figure 6C and D, we present the final three mice that were infected with the highest dose of LAINefdd and were followed for 5–6 months post infection. The viral loads for these mice were maintained below 106 copies LAINefdd RNA/ml blood throughout the experiment (Figure 6C). For all of these mice, we observed only small drops in peripheral blood CD4+ T cells (Figure 6D). The results are in contrast to the complete loss of CD4+ T cells observed as early as twenty-one days in mice infected with 600,000 TCIU of LAI and further establish the significantly attenuated pathogenic phenotype of LAINefdd. Consistent with the results in Figure 6D for peripheral blood, the long term infections also did not result in CD4+ T cell depletion in any of the organs analyzed- lymph node, spleen, bone marrow, lung, and liver (Figure 7, 6CD). Single and double positive thymocytes were also clearly resistant to depletion by LAINefdd infection (Figure 7, 6CD). In summary, the results indicate that the attenuated pathogenic phenotype of LAINefdd is not overwhelmed by a very high dose of virus.
The relationship between viral load, T cell activation, and the loss of peripheral blood CD4+ T cells and thymocytes
Relationship between Peak Viral Load, T cell depletion and CD4 + CD8 + Thymocyte Depletion a
Peak Viral Loadb(highest to lowest)
T cell Depletiond
2.04 × 107
1.19 × 107
1.13 × 107
6.70 × 106
5.63 × 106
4.99 × 106
4.64 × 106
4.25 × 106
4.24 × 106
3.77 × 106
3.59 × 106
3.29 × 106
2.07 × 106
1.56 × 106
1.25 × 106
1.02 × 106
9.93 × 105
8.82 × 105
4.90 × 105
4.36 × 105
3.00 × 105
2.31 × 105
2.20 × 105
1.91 × 105
7.73 × 104
6.49 × 104
A combined mechanism of T cell killing brought about by high viral replication plus reduced replacement of T cells by the thymus may account for the dramatic loss of T cells we have observed with LAI infection. In the case of the three LAINefdd-infected mice that lost peripheral blood T cells, there was no loss of CD4+CD8+ thymocytes which suggests that T cell replenishment may have been ongoing (Figure 7). This could account for the extended time course of T cell depletion for LAINefdd-12, LAINefdd-13 and LAINefdd-14 relative to that observed for LAI infections at the high viral dose (Figures 5B and 6B).
HIV-1 infection results in depression of thymic output of T cells . We have determined that deletion of the nef coding sequence prevents thymocyte loss by even a high dose of nef-defective virus. For T cell depletion, the cytoxcity of LAI appears to depend on reaching a threshold of replication not usually achieved by LAINefdd. Our results are consistent with Nef indirectly depleting T cells by elevating HIV-1 replication. However, CD4+CD8+ thymocytes appear to be depleted by a different mechanism in which Nef is acting as a pathogenic factor.
Recent reports have demonstrated the utility of the BLT humanized mouse model for investigating HIV-1 infection [24, 27, 33, 43, 47]. HIV-1 establishes a systemic infection with high viral loads that exhibits pathogenic properties by depleting human CD4+ T cells. Of particular interest to us was the extremely cytopathic phenotype of CXCR4-tropic viruses like HIV-1LAI. This well characterized and fully functional virus replicates to high levels in BLT mice and rapidly depletes CD4+ T cells from blood and tissues . The dramatic nature of this phenotype allowed us to investigate the role of Nef in two decisive phenotypes for the development of AIDS: maintenance of high levels of virus replication and depletion of CD4+ T cells and thymocytes [2, 49]. Our studies show that at low inoculums the nef-deleted HIV-1LAI was doubly defective with delayed replication and a severely blunted capacity to deplete CD4+ T cells and thymocytes. Mice infected with 3000 TCIU of wild-type LAI exhibited a viral load of 105 copies/ml of RNA 14 days post inoculation, but LAINefdd-infected mice only reached a viral load of 105 copies/ml at approximately 40, 40, 60, and 75 days post intravenous infection (Figure 3A). In addition, the low dose LAI-infected mice gave a 7-fold higher average peak viral load than the low dose LAINefdd-infected mice (Table 1). The pathogenic effect of Nef was also clearly demonstrated in Figure 3B, as LAI infection (3000 TCIU) resulted in an almost complete depletion of CD4+ T cells from peripheral blood, but LAINefdd infection resulted in at best a modest reduction in CD4+ T cells levels even after viral loads became elevated. Similar results were obtained at the ten-fold higher dose of 30,000 TCIU of LAI and LAINefdd (Figure 4). Thus, Nef is important, not only for efficient viral propagation, but also full pathogenicity in vivo.
At 600,000 TCIU of LAINefdd, CD4+ T cells were not depleted in seven of ten mice despite peak viral loads of 0.75 × 106 ± 0.25 × 106 demonstrating the great importance of Nef for pathogenicity. However, three of ten mice infected with the highest dose of nef-deleted virus were observed to have a pathogenic phenotype (Figure 5B). These three mice exhibited efficient viral replication as reflected by high peak viral loads (3.8 × 106 ± 0.2 × 106), but delayed reductions in CD4+ T cell levels. We attribute the loss of T cells in these mice to the high levels of replication of LAINefdd since no repair of the nef reading frame was observed. Repair of the defective nef was not expected, as all nef-defective HIV-1s from patients develop expanded deletions to include most of the nef coding sequence while sparing the polypurine tract [4, 7–9, 36].
Outcomes for patients infected with HIV-1 nef (−) for longer than 15 years a
Prior to 1983
Several Nef activities may be critical factors in the attenuation of LAI pathogenicity and replication that we observed. For example, we observed considerable delay in appearance of viral RNA in the blood for the lowest dose (Figure 3A). The loss of the enhancement of virion infectivity function could be significant early in infection to slow the rate of spread of the virus [37, 38]. Subsequently, following the delay in replication, higher levels of virus could reduce the impact of this effect and result in some LAINefdd-infected mice presenting with viral loads over 106. It is also important to determine the importance of another Nef activity, CD4 downregulation. Previous results from infections of SCID-hu thy/liv implants with Nefs mutated for residues (W57L58) critical for CD4 downregulation have given conflicting results. As a result, the importance of CD4 downregulation for replication and pathogenicity in vivo is unresolved [21, 22, 53]. The results presented here demonstrate that BLT mice have the potential to serve as an excellent model to address the roles of specific Nef functions described in vitro on Nef’s phenotype in vivo.
We did not observe loss of CD4+CD8+ thymocytes in LAINefdd infections despite virtually complete depletion of this same cell population by LAI. Further, it appeared that CD4+CD8- thymocytes were resistant to killing by LAI. This in vivo result is similar to the ex vivo result reported by Choudhary et al. who reported a greater cytotoxic effect by CXCR4-trophic HIV-1 on CD4+CD8+ than on CD4+CD8- thymocytes . This effect was attributed to CD4+CD8+, but not CD4+CD8- thymocytes, being primed for negative selection. Potentially consistent with the proposed susceptibility of CD4+CD8+ thymocytes to apoptosis, Nef has been reported to induce PD-1 and FasL [54, 55].
In summary, we have demonstrated that LAI lacking Nef function is greatly attenuated for pathogenicity. However, under supraphysiological conditions of infection, this linkage is not absolute; and the defective virus retains some capacity to systemically reduce CD4+ T cells. HIV-1 gene products other than Nef that may be responsible for the residual pathogenicity of LAINefdd are Vpr or Env [42, 43, 56]. The availability of an in vivo system to study the roles of HIV-1 accessory genes in viral replication and depletion of CD4+ T cells and thymocytes will help address currently unresolved questions. In particular, the mechanism of Nef induced killing of CD4+CD8+ thymocytes needs to be explored in future studies as there is evidence of an important role for HIV-1 inhibition of T cell replenishment from the thymus in disease progression [32, 57].
We have investigated the infection of BLT humanized mice by wild-type and nef-defective HIV-1. With wild type virus, there is rapid and near total depletion of CD4+ T cells in blood and in multiple tissue compartments. CD4+CD8+ thymocytes are also rapidly destroyed. With nef-defective virus, the course of infection is dramatically different. Despite the establishment of robust viral replication, there is rarely much loss of CD4+ T cells over the time frame that the wild-type virus systemically depletes these cells. With a high initial inoculum and an extended time course of infection, nef-defective virus may exhibit pathogenic properties. These observations are consistent with the conclusion that Nef is a replication factor and indirectly accelerates T cell killing by inducing high levels of viral replication. This mechanism does not apply to CD4+CD8+ thymocytes, and our results suggest that Nef has a direct role in thymocyte killing. The effectiveness of CXCR4-trophic HIV-1 in depleting CD4+ T cells may be enhanced by killing thymocytes and preventing thymic T cell replenishment.
Preparation of humanized BLT mice
Humanized BLT mice were prepared as previously described [24–27, 34, 58–60]. Briefly, thymus/liver implanted NOD/SCID or NOD/SCID IL-2γ−/− mice (The Jackson Laboratories, Bar Harbor, ME) were transplanted with autologous human CD34+ cells isolated from fetal liver (Advanced Bioscience Resources, Alameda, CA). Human reconstitution in the peripheral blood of these mice was monitored periodically by flow cytometry (FACSCanto; BD Biosciences). Mice were maintained either at the Animal Resources Center, UT Southwestern Medical Center at Dallas (UTSWMC) or at the Division of Laboratory Animal Medicine, University of North Carolina at Chapel Hill (UNC-CH) in accordance with protocols approved by the UTSWMC or UNC-CH Institutional Animal Care and Use Committee. To ensure genetic diversity, seventeen different tissue donors were used to generate three groups of mice used for the experiments presented in this manuscript. Specifically, the percent engraftment for five naïve mice was 49.8% ± 13.9% human CD45+ cells in blood from five of the donors, for the nine LAI-infected mice it was 40.3% ± 8.0% human CD45+ cells in blood from seven of the donors, and for seventeen LAINefdd-infected mice it was 51.4% ± 4.8% human CD45+ cells in blood from nine of the donors. Engraftment did not significantly differ between the groups. Peak level of virus in blood did not correlate with human cell engraftment.
Cell lines and culture conditions
293T and TZM-bl cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Cellgro, Herndon, VA) supplemented with 10% fetal bovine serum (FBS; Cellgro), 100 IU/ml of penicillin, 100 μg/ml streptomycin, and 2 mM glutamine (Cellgro) in 10% CO2 at 37°C.
The molecular clone, LAI (accession # K02013), is described by Peden et al.. pLAINefdd was made by first removing the internal XhoI and Acc65I fragment internal to nef, treating with Klenow and religating. This removes nucleotides 8495–8608, inclusive. The deleted LAI provirus was then cut at the reconstructed XhoI site, treated with Klenow and religated to introduce a frame shift (four base insertion) in addition to the deletion 114 bases. Second, nucleotides, 8692–8981, inclusive, were deleted by a site-directed mutagenesis strategy (Stratagene, USA). LAINefXhoI was constructed to be defective for nef by cutting the provirus with the single cutter XhoI, filling in with Klenow and religating. This leaves nef coding sequence intact but introduces a four-base frameshift at nef codon 35. Lack of Nef expression by the mutants was confirmed by Western blot analysis. Our LAI construct is comparable to the double deleted NL4-3 construct of Gibbs et al. . Converting NL4-3 numbers to homologous LAI numbers to compare the two constructs: the deletions were NL4-3/LAI (8428/8495 to 8650/8608 for the 5′ deletion and 8718/8692 to 8983/8981 for the 3′ deletion). This NL4-3 construct was used to produce virus for SCID-hu studies .
Virus production, exposure of BLT mice to HIV-1LAI or nef-deleted HIV-1LAI, tissue harvesting and cytometric analyses
Stocks of LAI, LAINefdd and LAIXhoI were prepared and titered as we previously described [62, 63]. Briefly, proviral clones were transfected into 293T cells. Viral supernatant was collected 48 hours after transfection and diluted in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 100 IU penicillin/ml, 100 μg/ml streptomycin, and 2 mM glutamine. TZM-bl cells were infected in 12-well tissue culture plates with 0.4 ml of virus containing medium for two hours. Then 1.0 ml of supplemented DMEM was added, and the plates were incubated overnight. Virus containing medium was removed the next day and the incubation was continued for 24 hours. The cells were fixed and stained (40 hours after first exposure to virus). The titers of these viral stocks were determined in triplicate and at least two different titer determinations were performed in each batch of virus used for all the experiments described in this manuscript. p24gag was determined for each prep of virus by ELISA.
Intravenous exposure of BLT mice with HIV-1LAI or HIV-1LAIdd was conducted via tail vein injection with 3000, 30,000 and 6 × 105 tissue culture infectious units (TCIU). Viral load in peripheral blood of infected mice was monitored longitudinally by quantitative real-time PCR using Taqman RNA to-CT™ 1-step kit from Applied Biosystems, USA [34, 64]. The sequences of the forward and reverse primers and the Taqman probe for PCR were: 5′- CATGTTTTCAGCATTATCAGAAGGA- 3′, 5′-TGCTTGATGTCCCCCCACT- 3′, and 5′- FAM CCACCCCACAAGATTTAAACACCATGCTAA- Q- 3′ , respectively. CD4+ and CD8+ T cell levels were monitored by flow cytometric analysis.
Flow cytometric immunophenotyping was performed on peripheral blood samples longitudinally and mononuclear cells were isolated from tissues at harvest. Whole peripheral blood from humanized mice was analyzed according to the BD Biosciences Lyse/Wash protocol (Cat. No. 349202) as we have previously described [24, 26, 27]. Briefly, following antibody labeling of whole blood, red blood cells were lysed. The remaining cells were washed, fixed; and the sample was analyzed by flow cytometry. Tissue mononuclear cell isolations and immunophenotyping analyses were also performed according to published methods [24, 26, 27]. Flow cytometric gating for all samples was performed as follows: (step 1) forward and side scatter properities were utilized to set a live cell gate; (step2) live cells were then analyzed for expression of the human pan-leukocyte marker CD45; (step 3) human leukocytes were then analyzed for hCD3, hCD4 and/or hCD8 expression (step 4) in the case of CD8+ T cell activation analyses, peripheral blood human CD8+ T cells were analyzed for hCD38 and HLA-DR expression.
Sequence analysis of plasma virions
Viral RNA was extracted from 20 μl of plasma from infected mice using the QIAamp Viral RNA Mini kit (Qiagen Sciences, USA). RNA was then reverse transcribed into cDNA, which was then subject to nested PCR. The outer primers for nef amplification are: 5′-AGCTTGCTCAATGCCACAGCC-3′ and 5′-GCTGCATATAAGCAGCTGCTTTTTG-3′. And the inner primers are: 5′-TAGAGCTATTCGCCACATACC-3′ and 5′-GCTTGCTACAAGGGACTTTCCGC-3′. Gel purified PCR products were sequenced and the sequences aligned to HIVLAI sequences to determine if sequence changes had occurred.
Log-rank (Mantel-Cox) tests (alpha level, 0.05) for initial viremia detection were performed using survival analyses in Prism version 4 (GraphPad). Unpaired two-tailed t tests and one-way ANOVA were also performed in Prism version 4 (Graph Pad). All data were plotted as mean ± S.E.M.
This work was supported by grant AI33331 from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health, USA and UNC CFAR P30 A1504410. Richard Watkins is supported by NIH Virology Training Grant 5T32A1007419 and Tomonori Nochi is supported by a Research Fellowship of the Japan Society for the Promotion of Science. We also acknowledge the advice we received from the UNC CFAR Statistics Core.
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