Quantification system for the viral dynamics of a highly pathogenic simian/human immunodeficiency virus based on an in vitroexperiment and a mathematical model

Table 1 Experimental data for the in vitro experiment

MOI	Measurement day
	0	1	2	3	4	5	6	7	8
Concentration of Nef-negative HSC-F cells (cells/ml)
2 × 10^-3	5470829	6044623	2690861	1012828	223584	42130	58470	10386	10270
2 × 10^-4	2333804	4953074	2985268	2201172	811240	621750	60255	19998	4857
2 × 10^-5	2574201	3563431	3434160	2345412	1269216	1345728	264794	71792	127996
2 × 10^-6	3357117	2583058	4557411	35074989	1334060	1896048	1022157	307908	153360
Concentration of Nef-positive HSC-F cells (cells/ml)
2 × 10^-3	d.l.*	d.l.	439139	1167172	736416	177870	41530	19614	9730
2 × 10^-4	d.l.	d.l.	84732	158828	548760	878250	89745	40002	5143
2 × 10^-5	d.l.	d.l.	d.l.	64588	170784	574272	165206	88208	92004
2 × 10^-6	d.l.	d.l.	d.l.	d.l.	65940	383952	347843	232092	86640
Total viral load of SHIV-KS661 (RNA copies/ml)
2 × 10^-3	9180000	331000000	2840000000	4050000000	3140000000	1120000000	154000000	20200000	5650000
2 × 10^-4	1030000	26200000	256000000	1670000000	2110000000	1740000000	609000000	134000000	19400000
2 × 10^-5	126744	4370000	51200000	489000000	1280000000	1940000000	1230000000	570000000	130000000
2 × 10^-6	10170	800536	4600000	54200000	322000000	1300000000	1210000000	603000000	275000000
Infectious viral load of SHIV-KS661 (TCID₅₀/ml)
2 × 10^-3	40	4064	40960	81920	163840	20480	2560	160	d.l.
2 × 10^-4	d.l.	101	403	5120	16255	40960	1280	101	40
2 × 10^-5	d.l.	64	640	4064	20480	25803	5120	1280	640
2 × 10^-6	40	40	80	640	5120	1280	1280	640	1280

"d.l." designates samples in which the concentration was below the detection limit.

ISSN: 1742-4690