Figure 2

Statistically significant reduction of tumor-free survival in Tax⁺p53^−/−mice compared to Tax⁺p53^+/+. (A) Tumor-free survival curves show decreased tumor free survival in Tax⁺p53^−/− mice compared to Tax⁺p53^+/− or Tax⁺p53^+/+ animals. Statistical significance (*: p<0.0001) between Tax⁺p53^−/− and either Tax⁺p53^+/− or Tax⁺p53^+/+ mice was determined using Gehan-Breslow-Wilcoxon test. (B) A Tax transgenic mouse with an ear tumor is shown for illustration. (C) Examples of tumor histology [hematoxylin and eosin (H&E) staining] from Tax⁺p53^+/+ mice are shown. Example of a pleomorphic ear sarcoma from a Tax⁺p53^+/+ mouse; (left): expansive soft tissue tumor located in peripheral connective tissue of the ear beneath the supportive cartilage; example of a hind leg pleomorphic histiocytic sarcoma and glandular adenocarcinoma with squamous hyperkeratosis from a Tax⁺p53^+/+ mouse (right): the sarcoma consisted primarily of histiocytic tumor cells with dispersed round cells, sparse spindle cells and neutrophilic granulocytes. Similar tumors are also seen in Tax⁺p53^−/− mice.