Figure 6

HERC5 restricts MLV Gag particle production. U2OS cells were co-transfected with pMLV-Gag and either empty plasmid, pHERC5 + CS, or pHERC5-C994A + CS with or without pUbp43. Gag particles released into the supernatant and intracellular Gag protein expression were analyzed by quantitative Western blotting using anti-MLV antisera or anti-β-actin 48 hours post-transfection. Numerical values on the blots display the densitometric quantification of the specified bands after normalization with β-actin levels.