Figure 6

Kinetics of RNA plasma viral load and mRNA levels of A3G and MxA in SIV-infected macaques. Plasma viral loads as well as A3G and MxA mRNA levels in PBMC were determined longitudinally in seven macaques before and after inoculation with SIV (left panels A-C). Viral load is depicted as log-transformed RNA copies per millilitre (ml) plasma (A). Relative A3G (B) mRNA and relative MxA mRNA (C) in PBMC were calculated as copy numbers per 100 copies of GAPDH. Data are expressed as fold increase over baseline after normalization to the mean of three preinfection values. Fine grey lines with symbols represent individual infected animals. Fine black lines with triangles depict the one animal inoculated but not infected. Bold lines show mean values of infected animals. The asterisks indicate a significant difference to the mean of the three preinfection values calculated by Mann-Whitney test (*p < 0.05). Right panels show relative mRNA levels of A3G (D) and MxA (E) in lymphocytes isolated from peripheral lymph nodes at selected time points. Each data point represents one individual animal with horizontal lines showing the median. For comparison the data from 10 dpi and 2 wpi were combined. Statistical analysis was calculated using the Kruskal-Wallis test with Dunn's multiple comparison test (*p < 0.05). pre, preinfection values; dpi, days post infection; wpi, weeks post infection.