SAMHD1: a new insight into HIV-1 restriction in myeloid cells
© St Gelais and Wu; licensee BioMed Central Ltd. 2011
Received: 21 June 2011
Accepted: 8 July 2011
Published: 8 July 2011
Human myeloid-lineage cells are refractory to HIV-1 infection. The Vpx proteins from HIV-2 and sooty mangabey SIV render these cells permissive to HIV-1 infection through proteasomal degradation of a putative restriction factor. Two recent studies discovered the cellular protein SAMHD1 to be this restriction factor, demonstrating that Vpx induces proteasomal degradation of SAMHD1 and enhances HIV-1 infection in myeloid-lineage cells. SAMHD1 functions as a myeloid-cell-specific HIV-1 restriction factor by inhibiting viral DNA synthesis. Here we discuss the implications of these findings in delineating the mechanisms of HIV-1 restriction in myeloid-lineage cells and the potential role of Vpx in lentiviral pathogenesis.
Myeloid-lineage cells, including monocytes, dendritic cells (DCs) and macrophages, play a multifaceted role in HIV-1 initial infection and viral dissemination; however, these cell types are restrictive to post-entry HIV-1 infection in vitro [1, 2]. For gene therapy purposes, transduction of human DCs with an HIV-1-derived lentiviral vector can be significantly enhanced by preincubation with virus-like particles derived from SIV . Subsequent studies indicated that Vpx proteins from sooty mangabey SIV (SIVsm) and HIV-2 lineages efficiently enhance HIV-1 infection in human DCs and promote the accumulation of full-length viral DNA . Further studies from several laboratories suggested that Vpx, similar to HIV-1 Vpr, interacts with the DCAF1 component of the CUL4A/DDB1 and E3 ubiquitin ligase complex (reviewed in [5, 6]). However, only SIVsm/HIV-2 Vpx can efficiently enhance HIV-1 infection in DCs and macrophages . These studies led to the hypothesis that Vpx targets a putative HIV-1 restriction factor for proteasomal degradation in myeloid cells through the E3 ubiquitin ligase complex [5, 6], prompting the search for the unknown HIV-1 restriction factor in human myeloid cells that is counteracted by Vpx.
SIVsm, SIVsm-derived rhesus macaque SIV (SIVmac), and HIV-2 encode both Vpr, a homologue of the HIV-1 Vpr protein, and Vpx, a protein unique to the SIVsm lineage. vpx has likely evolved via duplication of the primate lentivirus vpr gene . Early studies have demonstrated that macaques infected with Vpx-defective SIVmac or SIVsm had decreased viremia, impaired viral replication, and slower AIDS progression compared to wild-type SIV-infected animals, thus revealing the importance of Vpx in SIV pathogenesis [7, 8]. The important role of Vpx in lentiviral infection in myeloid-lineage cells in vitro and in vivo indicates that Vpx is not merely a functional copy of Vpr, but may possess a unique function. Although Vpx has been reported to facilitate nuclear import of viral DNA , the precise function of Vpx in lentiviral pathogenesis remains to be defined.
New findings and discussion
Using mass spectrometry, Laguette et al. identified SAMHD1 as a novel Vpx-interacting protein purified from differentiated human monocytic THP-1 cells that express tagged Vpx . The rationale for using THP-1 cells was based on the previous work that differentiated THP-1 cells can be rendered more permissive to HIV-1 infection by transduction of SIVsm/HIV-2 Vpx-containing virus-like particles derived from SIVmac . SAMHD1 is expressed in non-permissive cells, including THP-1 cells, primary monocytes, monocyte-derived macrophages and DCs, while permissive CD4+ T cells and monocytic U937 cells do not express endogenous SAMHD1 , suggesting an inverse correlation between SAMHD1 expression and permissiveness to HIV-1 infection. Moreover, silencing of SAMHD1 in non-permissive cells (THP-1 cells and DCs) alleviates HIV-1 restriction, and over-expression of SAMHD1 in permissive cells (HeLa cells and U937 cells) inhibits HIV-1 infection .
By contrast, Hrecka and colleagues identified SAMHD1 from HEK 293T cells expressing tagged Vpx in a proteomic screen using multidimensional protein identification technology . They demonstrated that Vpx relieves the inhibition of HIV-1 infection in monocyte-derived macrophages by mediating proteasome-dependent degradation of SAMHD1 through the CUL4A/DCAF1 E3 ubiquitin ligase . Both studies confirmed that Vpx interacts with SAMHD1 and induces proteasomal degradation of SAMHD1 in THP-1 cells or macrophages, which can be restored by treatment with a proteasome inhibitor [9, 10].
Comparison of HIV-1 and HIV-2 regarding SAMHD1 degradation and potential disease consequences.
Vpx protein expression
Human SAMHD1 degradation
Efficient infection of myeloid cells
Triggering myeloid-cell-mediated innate anti-viral immunity through type I interferon
Potential disease outcome
Spread of infection and AIDS
Confined infection, no AIDS in natural hosts
The biological function of SAMHD1 is largely unknown. SAMHD1 mutations are involved in Aicardi-Goutières syndrome (AGS), a genetic encephalopathy mimicking congenital viral infection . SAMHD1 was initially cloned from human DCs as an interferon (IFN)-γ-inducible gene  and has been proposed to act as a negative regulator of the IFN response . The cellular exonuclease TREX1 was recently shown to bind and digest excess cytosolic HIV-1 DNA that would otherwise activate type I IFN expression and trigger an innate immune response . Interestingly, similar to SAMHD1, TREX1 mutations in humans are associated with autoimmune and inflammatory diseases, including AGS . It is currently unknown whether polymorphisms of SAMHD1 and TREX1 are linked to AIDS progression or whether AGS patients are more susceptible to HIV-1 infection.
Three major retrovirus restriction factors have been identified: APOBEC3G, TRIM5α, and tetherin . HIV-1 has developed mechanisms of evading these restriction factors mainly through its accessory proteins, such as Vif for APOBEC3G and Vpu for tetherin. These restriction factors function across many different cell types, whereas SAMHD1 appears to be specific to the myeloid-lineage cells. It might be possible that SAMHD1 acts in concert with another myeloid-specific co-factor . It appears that only Vpx from the SIVsm/HIV-2 lineage counteracts SAMHD1-mediated HIV-1 restriction in myeloid cells [9, 10], while HIV-1 Vpr does not interact or degrade SAMHD1 . SAM domains are putative protein interaction modules that are capable of self-association and binding to RNA and non-SAM domain containing proteins. Given that SAMHD1 interferes with the accumulation of HIV-1 reverse transcripts, one can speculate that the SAM domain of SAMHD1 may bind HIV-1 RNA or proteins and mediate their degradation through the HD domain and the recruitment of the E3-ligase complex. Further delineation of the mechanisms of SAMHD1 restriction is required to fully understand the HIV-1 restriction in myeloid-lineage cells and why HIV-1 has not evolved a viral antagonist to counteract SAMHD1.
SAMHD1-mediated HIV-1 restriction has so far been analyzed only in monocyte-derived DCs  and macrophages , and it should be investigated in primary monocytes, myeloid DCs, as well as plasmacytoid DCs that can produce high levels of type I IFN upon HIV-1 stimulation [1, 2]. It is unclear whether SAMHD1 is also type I IFN inducible, similar to other HIV-1 restriction factors. It would be interesting to know whether SAMHD1 can restrict other retroviruses, endogenous retroviruses, or other non-retroviruses and whether viruses use their own viral components to counteract SAMHD1.
The discovery of SAMHD1 as a myeloid-cell-specific HIV-1 restriction factor opens many intriguing questions in understanding intrinsic immunity against HIV-1. When considering future therapeutic opportunities, enhancement of SAMHD1 function may help hosts develop potent innate and adaptive immune responses to HIV-1. Further investigation of the mechanisms underlying SAMHD1-mediated HIV-1 restriction will shed light on the innate immune response against retroviruses and aid in the future development of more effective anti-HIV-1 interventions.
List of abbreviations
human immunodeficiency virus type 1
human immunodeficiency virus type 2
simian immunodeficiency virus
sterile alpha motif domain- and HD domain-containing protein 1
damage-specific DNA binding protein 1
DDB1- and CUL4A-associated factor-1
apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3G
tripartite motif-containing protein 5α.
We thank members of the Wu laboratory for helpful discussions and critical reading of the manuscript. The research in the Wu laboratory is supported by grants (AI068493 and AI078762) to Li Wu from the NIH and by the program of Public Health Preparedness for Infectious Diseases (PHPID) of The Ohio State University. The authors apologize to all colleagues whose work has not been cited as a result of space limitations.
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