Figure 2

Transduction of 293T cells with chimeric MLV/HIV vectors packaged into HIV-1 viral particles. A and B. 293T cells were transduced with equivalent amounts of p24 capsid protein (50 ng), as determined for each of the indicated chimeric vectors. The influence of the HIV-1 Rev/RRE system, and 5' UTR cis elements, on transduction was assessed by fluorescence microscopy (A) and FACscan analysis (B) at 7 days post-transduction. C. 293T cells were transduced in the absence (No RT Inhibitor), or presence (+RT Inhibitor), of the HIV-1 specific non-nucleoside reverse transcriptase inhibitor, etravirine (100 nM). Transduced cells were assessed by fluorescence microscopy and FACscan analysis. D. The capacity of the MLV/HIV RRE + RU5PS vector to be stably maintained after 4 cell passages was examined by FACscan analysis. The percent GFP positive cells are indicated for each FACscan and 293T negative control (NC) cells are shown.