Figure 4
Vpx rescues HIV-1, but not SIV MAC or HIV-2, from the type I IFN response in MDDC. (A) MDDCs were treated with the indicated compounds for 24 h, and then challenged with VSV-G-pseudotyped, vpx+ or Δvpx SIVMAC GFP reporter virus. (B, C) MDDCs were treated with the indicated compounds for 24 h, then treated with either VSV-G-pseudotyped vpx+ or Δvpx SIVMAC-251 VLPs, and then challenged with either VSV-G-pseudotyped SIVMAC-239 (B) or HIV-1NL4-3 (C) GFP reporter viruses. (D, E) MDDCs were treated with LPS, then treated with either media or VSV-G pseudotyped HIV-1NL4-3 or SIVMAC-239 VLPs (containing all accessory genes) for 3 h, and then challenged with either VSV-G-pseudotyped SIVMAC-239 (D) or HIV-1NL4-3 (E) GFP reporter viruses. Data are representative of one of at least three independent experiments. Error bars represent ± SD (n = 3).