Volume 7 Supplement 1

16th International Symposium on HIV and Emerging Infectious Diseases

Open Access

Comparison of Cytotoxic activity and Interferon-g secretion by Natural Killer Cells in HIV-1 and HIV-2 infected individuals

  • Samuel Victor Nuvor1Email author,
  • Sarah Rowland-jones2,
  • Hilton Whittle3 and
  • Assan Jaye3
Retrovirology20107(Suppl 1):P9

DOI: 10.1186/1742-4690-7-S1-P9

Published: 11 May 2010

Background

The role of NK cells in slowing disease progression in HIV-2 infected individuals compare to HIV-1 infected individuals.

Methods

In this study peripheral blood mononuclear cells were obtained from 30 HIV-1 and 30 HIV-2 infected subjects from each of 3 categories of CD4 T-cell counts (>500, 200-500 and <200 cells/ul) together with 50 HIV uninfected control subjects. Lytic activity and IFN-g secretion by NK cells from HIV-1 and HIV-2 infected subjects were measured by chromium-release and ELISPOT assays respectively following incubation of PBMC with the NK-sensitive K562 cells. Viral load was also measured from the plasma samples of the subjects.

Results

The cytotoxic response by NK cells was significantly higher in HIV-2 than in HIV-1 infection in subjects with CD4-T cell count >500 cell/ul (p < 0.05) and was similar to that of the healthy controls. There was a significant correlation between the magnitude of the NK population and cytolytic activity in HIV-2 individuals (r = 0.27, p = 0.01). There was also an inverse relationship between the cytolytic activity and plasma viral load in HIV-2 infected subjects (r = -0.27, p = 0.009). Interferon-g secretion by NK cells in ELISPOT assays was similar in HIV-1 and HIV-2 infections at all categories of CD4+T cell counts.

Discussion

The data suggest an efficient cytolytic function from NK cells in early HIV-2 infection, which is associated with high CD4 T cell counts. This may imply that a strategic immune-based therapy to control HIV disease through the enhancement of NK cell activity is worthy of consideration.

Authors’ Affiliations

(1)
University of Cape Coast
(2)
Wetherall Institute of Molecular Medicine
(3)
Medical Research Council

Copyright

© Nuvor et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

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