Table 2 Primers used in this work
Name | Function | Position in HXB22 | Sequence (5' to 3') |
|---|---|---|---|
O_envf | env PCR OF1 | 5964-5984 | TYTCCTATGGCAGGAAGAAGC |
O_envr | env PCR OR | 9096-9074 | TAACCCWTCCAGTCCCCCCTTTT |
MO130 | env PCR IF | 6207-6228 | GAGCAGAAGACAGTGGCAATGA |
MO147 | env PCR IR | 8834-8810 | CATCCMACTATRCTRCTTTTTGACC |
MO150 | env sequencing | 6976-6955 | ATTCCATGTGTACYTTGTACTG |
MO151 | env sequencing | 6859-6880 | CAATTCCCATACATTATTGTGC |
MO152 | env sequencing | 7668-7647 | CACTTCTCCAATTGTCCRTCAT |
MO153 | env sequencing | 7516-7537 | GACAAGCAATGTATGCCCCTCC |
MO154 | env sequencing | 8241-8220 | ACCAATTCCACAYACTTGCCCA |
MO155 | env sequencing | 8050-8072 | CTGGAACKCTAGTTGGAGTAAT |
MO042 | gag p24 OF-1 | 890-909 | TAGTATGGGCAAGCAGGGAG |
MO024 | gag p24 OF-2 | 508 - 527 | AACCCACTGCTTAAGCCTCA |
MO044 | gag p24 OR | 2272-2252 | TGCCAAAGAGTGATTTGAGGG |
MO043 | gag p24 IF | 1048-1067 | TGYGTRCATCAAARGATAGA |
MO045 | gag p24 IR | 2118-2101 | CCCCTTGYTGGAAGGCCA |
MO034 | 5' LTR to gag p24 OF | 478 - 479 | TGAGCCTGGGAGCTCTCTG |
MO186 | p24 to env OF | 1958 - 1985 | TTAARTGTTTCAACTGTGGCAAAGAAGA |
MO187 | p24 to env OR | 6420 - 6445 | CAAGCATGKGTAGCCCAGAYATTATG |
MO188 | p24 to env IF | 2034 - 2060 | ATGTGGGAARGARGGACACCAAATGAA |
MO189 | p24 to env IR | 6335 - 6360 | TCCACACAGGTACCCCATAATAGACT |
MO191 | 5' LTR to gag p24 OR | 832 - 859 | AATGCTGWRAACATGGGTATTACTTCTG |
MO192 | 5' LTR to gag p24 IR | 786 - 814 | TCTATTACTTTYACCCATGCATTTAAAGT |
MO193 | env to 3' LTR IF | 7922 - 7944 | CAGACCCTTATCCCAAACCCAAC |
MO194 | env to 3'LTR IR | 8606 - 8629 | CCCCCCTTTTCTTTTAAAAAGWRGC |
AJB-1R | p24 to env sequencing | 2239 - 2262 | TATGGATTTTCAGGYCCAATTYTTG |
AJB-2F | p24 to env sequencing | 2036 - 2058 | GCCCAAARGTTAAACAATGGCCA |
AJB-3R | p24 to env sequencing | 2846 - 2871 | TTCTGTATRTCATTGACAGTCCAGCT |
AJB-4F | p24 to env sequencing | 2741 - 2765 | ACACCAGAYAARAARCATCAGAAAG |
AJB-5R | p24 to env sequencing | 3585 - 3610 | GATTCCTAATGCATACTGTGAGTCTG |
AJB-6F | p24 to env sequencing | 3585 - 3610 | CAGACTCACAGTATGCATTAGGAATC |
AJB-7R | p24 to env sequencing | 3722 - 3750 | ACTAATTTATCTACTTGTTCATTTCCGCC |
AJB-8R | p24 to env sequencing | 4357 - 4383 | ATGTCTAYTATTCTTTCCCCTGCACTG |
AJB-9F | p24 to env sequencing | 4196 - 4219 | ATTCCCTACAATCCCCAAAGMCARG |
AJB-10F | p24 to env sequencing | 4609 - 4633 | TGATTGTGTGGCARGTAGACAGGAT |
AJB-11R | p24 to env sequencing | 4830 - 4854 | TCCATTCTATGGAGACYCCMTGACC |
AJB-12R | p24 to env sequencing | 5498 - 5521 | TGCCATAGGARATGCCTAAGCCYTT |
AJB-13F | p24 to env sequencing | 5498 - 5521 | AARGGCTTAGGCATYTCCTATGGCA |