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Figure 2 | Retrovirology

Figure 2

From: Identification of host proteins associated with HIV-1 preintegration complexes isolated from infected CD4+ cells

Figure 2

Isolation of HIV-1 PICs and their activity. (A) Magnetic separation of functional HIV-1 PICs. The PIC integrates HIV-1 DNA into the ~1.5 kb biotinylated non-viral DNA from pNL4-3 plasmid that serves as a target DNA in the in vitro assay. The biotin-target DNA-protein complex is then isolated using streptavidin magnetic beads after incubation at room temperature (RT) for 30 minutes. (B) Integration activity of HIV-1 PICs. The integration activity of the PICs isolated from SupT1/H9-HTLVIIIB cell co-cultures (HIV-1-infected, set to 100 percent) and control cytoplasmic extract from SupT1/H9 cell co-cultures (control cells), using biotin-target DNA, is shown. The analysis was performed as described previously [5].

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Retrovirology

ISSN: 1742-4690

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