Figure 4
From: Changes in the accessibility of the HIV-1 Integrase C-terminus in the presence of cellular proteins
Masking of the IN C-terminus in infected cells. (A) Streptavidin paramagnetic beads capture (SA capture) (1,2,3) or LEDGF/p75 co-immunoprecipitation (4,5) of the biotinylated IN from extracts of 293 cells mock-transduced (lane 1) or transduced with the IN-BAD vector (293 IN-BADv) (lane 2, 4) or INQ168A-BAD vector (293 INQ168A-BADv (lane 3, 5) analysed by Western blotting with the anti-IN (top) or anti LEDGF/p75 (bottom) antibodies (3 minutes exposure). (B) LEDGF/p75 co-immunoprecipitation or streptavidin capture of the biotinylated integrase from extracts of 293ctl (ctl) or 293sh (sh) mixed with the IN-BAD vector (IN-BADv). As a control, equal amount of 293ctl or 293sh lysates were tested for beta-actin content by WB (bottom panel).