Figure 4

Interactions of the ZAP mutants with the RNA helicase p72. Bacterially expressed GST or GST-p72 was immobilized onto glutathione-Sepharose 4B resin. The resins were washed and incubated with cell lysates of the NZAP-Zeo-myc mutants in the presence of RNase A for 2 h. The resins were washed and boiled in the sample loading buffer. The proteins were resolved by SDS-PAGE and detected by Western blotting using the anti-myc antibody. Input: total cell lysate.