Dominance of highly divergent feline leukemia virus A progeny variants in a cat with recurrent viremia and fatal lymphoma

Table 2 Oligonucleotides used in this study

Assay/Application	Oligonucleotide	Sequence	Amplicon size (bp)	Nucleotide position (bp)
TaqMan^® PCR assay
env variant¹	Forward	GAT CCG GAC CGA CCA TAA TTA A	105	1,912 - 1,933
	Probe	TGT ATG ATT CCA TTT AGT CCC⁶		1,935 - 1,955
	Reverse	ACA CCA CTG CAG TAG CTG GCT AA		2,017 - 1,995
Production of standard
FeLV-A/Glasgow-1²	Forward	TGG GGC CAA AGG GAA CAC AT	598	456 - 475
	Reverse	GTT ACC TAA GAT TGC AAT CCC TTC G		1,054 - 1,030
env variant³	Forward⁵	CCT ATG GCT CAC TTC TTT GAT ACT GAT ATC TCT A	2,664⁷	5,617 - 5,650
	Reverse	TTA TAG CAG AAA GCG CGC G		8,281 - 8,263
In situ hybridization⁴
p27	Forward	TAC GCC TTT ATC GCC AGT TG	342	1,840 - 1,859
	Reverse	ATC TTT CTT CCC TTT CCT CTG G		2,181 - 2,160
gp70	Forward	AGG GAT TGC AAT CTT AGG TA	219	6,952 - 6,971
	Reverse	TTA CAG GCC CAA TAG GTG		7,170 - 7,153

¹ based on FeLV SP261-III [GenBank: EU359305]; ² based on FeLV-A/Glasgow-1 [GenBank: M12500]; ³ based on FeLV-A Rickard strain [GenBank: AF052723], ⁴ based on FeLV-FAIDS [GenBank: M18247]; ⁵ [29]; ⁶ 5' FAM/3' TAMRA; ⁷ the PCR product included the first 300 nucleotides of the LTR in addition to env

ISSN: 1742-4690