Figure 5
From: Anti-tetherin activities in Vpu-expressing primate lentiviruses
Specificity of interaction between SIVcpz and SIVmac Nef proteins and tetherin. (A) Partial sequence alignment of human, chimpanzee (Cpz) and H(+5)-tetherin proteins, showing the N-terminal cytoplasmic tail, the transmembrane (TM) domain and the start of the extracellular domain. H(+5)-tetherin contains an insertion (DDIWKK) from Cpz-tetherin in place of human tetherin residue E-14. (B) Western blot of expression of indicated tetherin constructs, from lysates of transfected 293A cells. (C) Anti-tetherin activities of SIVmac239 and SIVcpz Nef-EGFP proteins against H(+5)-tetherin expressed in 293A cells. Mean fold-enhancement of HIV-1 VLP release in presence of Nef-EGFP proteins is shown relative to baseline (control) levels for n = 2 independent experiments.