Figure 1

Gag CM9 Qdot 655 multimer validation. A) Flow cytometry analysis of Gag CM9 specific CD8⁺ T cell clones showed that > 99% of the cells bound to the Gag CM9 Qdot 655 multimer or to the Gag CM9 APC tetramer. The multimer and the tetramer are coupled with the same Gag CM9 monomers. ≤ 0.13% of Gag CM9 specific cells bound to the negative control FLP Qdot 655 multimer. B) Flow cytometry analysis of PBMCs and single cell suspension of lymph nodes demonstrated that a distinct population of CD3⁺CD8⁺ cells, 1.74% in blood and 3.82% in lymph node single cell suspension, from SIV infected Mamu-A*01 positive RM bound the Gag CM9 Qdot 655 multimer. ≤ 0.17% of CD3⁺CD8⁺ cells from Mamu-A*01 positive RM that were not SIV infected or cells from Mamu-A*01 negative RM that were either SIV infected or uninfected bound the Gag CM9 Qdot 655 multimer. ≤ 0.31% of CD3⁺CD8⁺ cells bound the FLP Qdot 655 multimer. The gating strategy was as described in Methods. ND; not done due to lack of material.