Figure 3

Breadth of sCD4- 40 -17b activity against pseudotypes from genetically diverse HIV-1 primary isolates. Nearly all entries are primary isolates for which the Env sequences were originally obtained by direct cloning from infected tissue; the exceptions are the laboratory-adapted Ba-L, and 89.6 strains. The IC₅₀ values (μg/ml) represent the mean of multiple independent assays (+/- SEM) (3-5 replicate assays in most cases, 2 in a few instances). The values are color coded according to the IC₅₀ ranges as indicated at the top of the figure, with red<orange<yellow<white. Note that none of the IC₅₀ values are in the white range (least potent). The superscript letters indicate the references describing the individual isolates, as follows: ^a[41], clade A; ^b[39], clade B, tier 2 reference panel; ^c[40], clade C reference panel; ^d [V. Polonis and S. Tovanabutra, personal communication], clades A, D, AE and AG. Further details on various features of these isolates can be found in these cited references. ** Indicates isolates previously found to be insensitive to sCD4-17b in the MAGI-CCR5 assay using infectious virus [29].