Figure 1
From: Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV-like sequences
Sensitivity of TaqMan qPCR for IN region in XMRV pol. Titration of DNA from WPI-1282 (1.7, 16.7, and 166.7 cell equivalents) in the absence (square, solid line, slope = -3.14) or in the presence of 8.3 × 105 cell equivalents of genomic LNCaP DNA (circle, dotted line, slope = -3.04). 1.7 cell equivalents of WPI-1282 genomic DNA is detectable in 8.3 × 105 cell equivalents of background DNA. Samples were run in duplicates. All qPCR reactions were run for 60 cycles. Samples that did not produce a signal after 60 cycles were assumed negative for XMRV. Ct = Cycle Threshold