Mouse DNA contamination in human tissue tested for XMRV
- Mark J Robinson†1,
- Otto W Erlwein†1,
- Steve Kaye1,
- Jonathan Weber1,
- Oya Cingoz2,
- Anup Patel3,
- Marjorie M Walker4,
- Wun-Jae Kim5,
- Mongkol Uiprasertkul6,
- John M Coffin2 and
- Myra O McClure1Email author
© Robinson et al; licensee BioMed Central Ltd. 2010
Received: 18 November 2010
Accepted: 20 December 2010
Published: 20 December 2010
We used a PCR-based approach to study the prevalence of genetic sequences related to a gammaretrovirus, xenotropic murine leukemia virus-related virus, XMRV, in human prostate cancer. This virus has been identified in the US in prostate cancer patients and in those with chronic fatigue syndrome. However, with the exception of two patients in Germany, XMRV has not been identified in prostate cancer tissue in Europe. Most putative associations of new or old human retroviruses with diseases have turned out to be due to contamination. We have looked for XMRV sequences in DNA extracted from formalin-fixed paraffin- embedded prostate tissues. To control for contamination, PCR assays to detect either mouse mitochondrial DNA (mtDNA) or intracisternal A particle (IAP) long terminal repeat DNA were run on all samples, owing to their very high copy number in mouse cells.
In general agreement with the US prevalence, XMRV-like sequences were found in 4.8% of prostate cancers. However, these were also positive, as were 21.5% of XMRV-negative cases, for IAP sequences, and many, but not all were positive for mtDNA sequences.
These results show that contamination with mouse DNA is widespread and detectable by the highly sensitive IAP assay, but not always with less sensitive assays, such as murine mtDNA PCR. This study highlights the ubiquitous presence of mouse DNA in laboratory specimens and offers a means of rigorous validation for future studies of murine retroviruses in human disease.
In 2006, XMRV was identified in stromal cells associated with prostate cancers of men with a family history of the malignancy, 40% of whom were homozygous for a specific variant of the interferon-inducible RNaseL gene, suggesting an increased susceptibility to viral infection in these patients. Virochip microarray analysis on cDNA from some of these tumours led to the identification of XMRV , a gammaretrovirus closely related, but not identical, to endogenous MLV. Interest in XMRV intensified when 6% of all prostate cancers in a US clinic were found to carry the virus and when by immuno-histochemical staining the virus was detected in the tumour epithelium of 23% of those patients. In the latter study, virus detection was associated with a higher Gleason Index and appeared to be independent of the RNAseL mutation . Several papers have since demonstrated a link of prostate cancer with XMRV [3–5], however this has not been repeated in other cohorts [6–9].
XMRV has also been reported in patients suffering from chronic fatigue syndrome (CFS) , a condition also associated with perturbations in the RNaseL innate defence response. In addition, XMRV has been described in the bronchiolar lavages of immunosuppressed individuals . Several groups have presented unambiguous data challenging the original findings, both in European CFS cohorts [12–14] and in US CFS patients [15, 16]. More recently, matters were further complicated by the publication of a study finding gag sequences similar to those of four different polytropic endogenous MLVs in an unrelated US CFS cohort, but no evidence of XMRV . A clear account of the claims and counter-claims surrounding XMRV and its disease association has recently been published .
The UK prostate specimens in this study came from two sources. Cancer tissue came from men locally referred to St Mary's Hospital in West London with symptoms of voiding dysfunction and prostate specific antigen abnormality and requiring biopsy after appropriate counselling. In addition, some patients had participated in a voluntary screening study and these provided samples that were a mixture of benign and cancer pathologies. All biopsy tissue had been stored in formalin fixed, paraffin-embedded (FFPE) blocks over a period of 3-6 years. Slices were taken from the blocks and DNA extracted as described (see Additional File 1; Supplementary Methods). The FFPE samples from Thailand and Korea were excess tissue from histological samples taken from new cases of both benign prostate hyperplasia and prostate cancer. These were FFPE embedded and sent to London for analysis.
We investigated the prevalence of XMRV in the UK and in the Far East, aware that the close relationship (about 94% at the nucleotide level) to other murine exogenous and endogenous retroviruses posed a problem in distinguishing XMRV from contaminating mouse DNA sequences. We were further aware that in any retrovirology laboratory MLV sequence contamination is something of an occupational hazard . For these reasons, we extracted the DNA from FFPE prostate cancers, along with benign hyperplasia tissue, and PBS without tissue. We used several sets of primers  to test for XMRV-specific sequences, derived from the XMRV gag leader  which encompasses the 24 bp deletion originally thought to distinguish XMRV as a new human virus. To control for low level contamination, we included multiple no-template controls (no less than 6 in every run) and included assays with primers that would amplify murine mitochondrial DNA (mtDNA) and intracisternal A particle (IAP) LTRs. IAPs are retrotransposons present at the level of about 1000 copies of varying length per mouse genome .
All murine retroviral primer sequences amplified specific products of the appropriate size when tested on pXMRV isolate VP62, an infectious molecular clone of XMRV, kindly provided by R. Silverman. The IAP primers did not amplify sequences from human DNA extracted from LNCaP cells (prostate cancer cell line) or from six PBMC samples from human prostate cancer patients (data not shown).
All FFPE prostate cancer specimens from the Pathology Department at St Mary's hospital London were provided to us in batches. The tissue slices were coded and assayed blind. Initially, the PCR amplification and sequence analysis of the amplicons encouraged us to deduce that we had detected a genuine XMRV infection in some of the samples. When on unblinding we found a concordant result from the same patient whose duplicate specimens had been provided in different batches, this appeared to reaffirm a genuine XMRV infection. Moreover, in two patients in whom the tumour was unilateral, XMRV was detected only in the cancerous lobe. Taken together with the consistently negative PCR water controls, the probability of contamination appeared to be low.
Frequency of positive PCR reactions using XMRV LTR primers, mtDNA primers and IAP primers.
(1 † )
XMRV shares extensive sequence identity with known xenotropic, nonecotropic and polytropic murine viruses; the first of which is known to infect many common human tumour cell lines, a phenomenon that has confused retrovirologists looking for disease associations for over three decades. Most putative associations of new or old human retroviruses with diseases (including CFS and prostate cancer) have turned out to be laboratory artefacts . The case of XMRV as a new human pathogen must be judged against this background . It is true that we cannot formally rule out the possibility that the samples in question are infected with XMRV and simultaneously contaminated by mouse DNA, although this is unlikely since we found no IAP-negative samples from which we amplified MLV-specific sequences (data not shown). Also, the failure to detect XMRV sequences other than in association with mouse DNA contamination in our cohort does not mean that the virus is not present in other, unrelated, cohorts.
It is difficult to explain how the contamination may have occurred, especially since the samples came from three unrelated centres in the UK, Korea and Thailand. As both our negative tissue and PBS controls treated in parallel with the FFPE were XMRV PCR-negative, it is unlikely that contamination was introduced via reagents. The UK FFPE tissue samples were stored boxed and stacked in a cupboard in the histopathology department for several years; and it is possible that contamination happened during that time, although why only a few samples (4.8%) were XMRV positive and the remainder not is difficult to explain. Nor does it explain the Thai and Korean results on tissue collected prospectively for the study. It does, however, demonstrate the necessity of controlling by highly specific and sensitive means for mouse contamination.
We are grateful for support of this work from the NIHR Biomedical Research Centre funding scheme. We should like to thank Professor Robin Weiss for critical reading of the manuscript. JMC was supported by grant R37 CA 089441 from the National Cancer Institute and a Research Professorship from the American Cancer Society with support from the George M Kirby Foundation.
- Urisman A, Molinaro RJ, Fisher N, Plummer SJ, Casey G, Klein EA, Malathi K, Magi-Galluzzi C, Tubbs RR, Ganem D, Silverman RH, DeRisi JL: Identification of a novel gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variant. PLos Pathog. 2006, 2: 211-225. 10.1371/journal.ppat.0020025.View Article
- Schlaberg R, Choe DJ, Brown KR, Thaker HM, Singh IR: XMRV is present in malignant prostatic epithelium and is associated with prostate cancer, especially high-grade tumors. Proc Natl Acad Sci USA. 2009, 106: 16351-16356. 10.1073/pnas.0906922106.PubMed CentralView ArticlePubMed
- Danielson BP, Ayala GE, Kimata JT: Detection of Xenotropic Murine Leukemia Virus-Related Virus in Normal and Tumor Tissue of Patients from the Southern United States with Prostate Cancer is Dependent on Specific Polymerase Chain Reaction Conditions. J Inf Dis. 2010, 202: 1470-1477. 10.1086/656146.View Article
- Hong S, Klein EA, Das Gupta J, Hanke K, Weight CJ, Nguyen C, Gaughan C, Kim KA, Bannert N, Kirchhoff F, Munch J, Silverman RH: Fibrils of prostatic acid phosphatase fragments boost infections with XMRV (xenotropic murine leukaemia virus-related virus), a human retrovirus associated with prostate cancer. J Virol. 2009, 83 (14): 6995-7003. 10.1128/JVI.00268-09.PubMed CentralView ArticlePubMed
- Arnold RS, Makarova NV, Osunkoya AO, Suppiah S, Scott TA, Johnson NA, Bhosle SM, Liotta D, Hunter E, Marshall FF, Ly H, Molinaro RJ, Blackwell JL, Petros JA: XMRV infection in patients with prostate cancer: novel serologic assay and correlation with PCR and FISH. Urology. 2010, 75 (4): 755-61. 10.1016/j.urology.2010.01.038.View ArticlePubMed
- Aloia AL, Sfanos KS, Isaacs WB, Zheng Q, Maldarelli F, De Marzo AM, Rein A: XMRV: A new virus in prostate cancer?. Cancer Res. 2010,
- Fischer N, Hellwinkel O, Schulz C, Chun FK, Huland H, Aepfelbacher M, Schlomm T: Prevalence of human gamma retrovirus XMRV in sporadic prostate cancer. J Clin Virol. 2008, 43: 277-283. 10.1016/j.jcv.2008.04.016.View ArticlePubMed
- D'Arcy F, Foley R, Perry A, Marignol L, Lawler M, Gaffney E, Watson RGW, Fitzpatrick JM, Lynch TH: No evidence of XMRV in Irish prostate cancer patients with the R462Q mutations. European Urology Suppl. 2008, 7: 271. 10.1016/S1569-9056(08)60798-8.View Article
- Hohn O, Krause H, Barbarotto P, Niederstadt L, Beimforde N, Denner J, Miller K, Kurth R, Bannert N: Lack of evidence for xenotropic murine leukemia virus-related virus (XMRV) in German prostate cancer patients. Retrovirology. 2009, 6: 92. 10.1186/1742-4690-6-92.PubMed CentralView ArticlePubMed
- Lombardi VC, Ruscetti FW, Das Gupta J, Pfost MA, Hagen KS, Peterson DL, Ruscetti SK, Bagni RK, Petrow-Sadowski C, Gold B, Dean M, Silverman RH, Mikovits JA: Detection of an infectious retrovirus, XMRV, in blood cells of patients with chronic fatigue syndrome. Science. 2009, 326: 585-589. 10.1126/science.1179052.View ArticlePubMed
- Fischer N, Schulz C, Stieler K, Hohn O, Lange C, Drosten C, Aepfelbacher M: Xenotropic murine leukemia virus-related gammaretrovirus in respiratory tract. Emerg Infect Dis. 2010, 16: 1000-1002.PubMed CentralView ArticlePubMed
- Erlwein O, Kaye S, McClure MO, Weber J, Wills G, Collier D, Wessely S, Cleare A: Failure to detect the novel retrovirus XMRV in chronic fatigue syndrome. PLoS ONE. 2010, 5: e8519. 10.1371/journal.pone.0008519.PubMed CentralView ArticlePubMed
- van Kuppeveld FJ, de Jong AS, Lanke KH, Verhaegh GW, Melchers WJ, Swanink CM, Bleijenberg G, Netea MG, Galama JM, van der Meer JW: Prevalence of xenotropic murine leukaemia virus-related virus in patients with chronic fatigue syndrome in the Netherlands: retrospective analysis of samples from an established cohort. BMJ. 2010, 340: c1018. 10.1136/bmj.c1018.PubMed CentralView ArticlePubMed
- Groom HCT, Boucherit VC, Makinson K, Randal E, Baptista S, Hagan S, Gow JW, Mattes FM, Breuer J, Kerr JR, Stoye JP, Bishop KN: Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome. Retrovirology. 2010, 7: 10. 10.1186/1742-4690-7. 10.PubMed CentralView ArticlePubMed
- Jia H, Hohn O, Zheng H, Tang S, Shankar A, Bannert N, Simmons G, Hendry RM, Falkenberg VR, Reeves WC, Heneine W: Absence of evidence of xenotropic murine leukemia virus-related virus infection in persons with chronic fatigue syndrome and healthy controls in the United States. Retrovirology. 2010, 7: 57. 10.1186/1742-4690-7-57.PubMed CentralView ArticlePubMed
- Henrich TJ, Li JZ, Felsenstein D, Kotton CN, Plenge RM, Pereyra F, Marty FM, Lin NH, Grazioso P, Crochiere DM, Eggers D, Kuritzkes DR, Tsibris AMN: Xenotropic Murine Leukemia Virus-Related Virus Prevalence in Patients with Chronic Fatigue Syndrome or Chronic Immunomodulatory Conditions. J Inf Dis. 2010, 202: 1478-1481. 10.1086/657168.View Article
- Lo SC, Pripuzova N, Li B, Komaroff AL, Hung GC, Wang R, Alter HJ: Detection of MLV-related virus gene sequences in blood of patients with chronic fatigue syndrome and healthy blood donors. Proc Natl Acad Sci USA. 2010, 107 (36): 15874-15879. 10.1073/pnas.1006901107.PubMed CentralView ArticlePubMed
- Kearney M, Maldarelli F: Current Status of Xenotropic Murine Leukemia Virus-Related Retrovirus in Chronic Fatigue Syndrome and Prostate Cancer: Reach for a Scorecard, Not a Prescription Pad. J Inf Dis. 2010, 202: 1463-1466. 10.1086/657169.View Article
- Voisset C, Weiss RA, Griffiths DJ: Human RNA "Rumor" Viruses: the Search for Novel Human Retroviruses in Chronic Disease. Microbiol & Mol Biol Reviews. 2008, 72: 157-196. 10.1128/MMBR.00033-07.View Article
- Dupressoir A, Heidmann T: Expression of intracisternal A-particle retrotransposons in primary tumors of oncogene-expressing transgenic mice. Oncogene. 1997, 14: 2951-2958. 10.1038/sj.onc.1201148.View ArticlePubMed
- Courgnaud V, Battini J, Sitbon M, Mason AL: Mouse retroviruses and chronic fatigue syndrome: Does X (or P) mark the spot?. PNAS. 2010, 107 (36): 15666-15667. 10.1073/pnas.1007944107.PubMed CentralView ArticlePubMed
- Weiss RA: A cautionary tale of virus and disease. BMC Biol. 2010, 8: 24. 10.1186/1741-7007-8-124.View Article
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