Figure 3

Ferroportin expression has no effect on transcription from CMV promoter. 293T cells were transiently transfected with CD4 expression vector, c-Myc-tagged WT ferroportin-expression vector or mutant c-Myc-tagged C326Y ferroportin -expression vector and also co-transfected with CMV-EGFP expression vector. A, photographs of cells on Olympus CKX41 using blue filter for EGFP fluorescence or phase contrast. B, EGFP fluorescence of cell lysates was measured on Luminescence spectrometer.