Volume 6 Supplement 3

AIDS Vaccine 2009

Open Access

P02-04. Multimeric soluble CD40 ligand efficiently enhances HIV specific cellular immune responses during DNA prime and boost with attenuated poxvirus strains

  • J Najera1,
  • C Gomez1 and
  • M Esteban1
Retrovirology20096(Suppl 3):P9

DOI: 10.1186/1742-4690-6-S3-P9

Published: 22 October 2009

Background

The attenuated poxvirus vectors MVA and NYVAC are now in clinical trials against HIV/AIDS. Due to the vectors restricted replication capacity in human cells, approaches to enhance their immunogenicity are highly desirable.

Methods

Here, we have analyzed the ability of a soluble form of hexameric CD40L (sCD40L) to stimulate specific immune responses to HIV antigens when inoculated in mice during priming with DNA and in the booster with MVA or NYVAC, expressing the vectors HIV-1 Env, Gag, Pol and Nef antigens from clade B.

Results

Our findings revealed that sCD40L in DNA/poxvirus combination enhanced the magnitude about 2-fold (DNA-B/MVA-B) and 4-fold (DNA-B/NYVAC-B), as well as the breath of the HIV antigen specific cellular immune responses. sCD40L was necessary in both prime and boost inoculations triggering a potent polarization of the the response towards a Th1 type. In DNA-B/NYVAC-B regime the addition of sCD40L significantly enhanced the humoral immune response against HIV gp160, but not in DNA-B/MVA-B combination.

Conclusion

These findings provided evidence for the immunostimulatory benefit od sCD40L when DNA and the poxvirus vectors MVA and NYVAC are used as immunogens.

Authors’ Affiliations

(1)
Molecular and Cellular Biology, Centro Nacional de Biotecnología-CSIC

Copyright

© Najera et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

Advertisement